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The invention relates generally to the field of dielectrophoresis, and more particularly to the field of the dielectrophoretic orientation of biological organisms.
2. Background Art
A variety of techniques exist in the fields of analytical chemistry, biochemistry and biology, as well as in related fields of electrochemistry, electroanalysis and microscopy, for separating and orienting particles. Among these techniques are the dielectrophoretic, electrophoretic and magnetic techniques. For example, the dielectrophoretic technique is based on the interactions between particulate matter and various substances having an electrical dipole moment, such as viruses and bacteria, which are suspended in a fluid such as salt water. The particles are placed in an electric field at right angles to the direction of flow of the fluid and the particles are separated by the force resulting from the electrostatic and magnetic dipole moments developed in the suspended particles as they are exposed to the electric field. In a similar manner, bacteria and viruses may be separated by an alternating magnetic field.
The electrophoretic technique is based on the phenomena associated with particles within a fluid medium as the particles are exposed to an electric field. As the electric field is applied, the particles move away from the field at a specific rate, which depends on the physical properties of the particles, the electrical properties of the medium in which the particles are suspended, as well as on other factors. If two or more types of particles are suspended in the medium, they are separated from one another in accordance with their physical and/or electrical properties.
Within the context of such techniques, and particularly the dielectrophoretic technique, it is conventional to utilize stationary carrier techniques in order to generate a moving suspension of particles within a fluid. For example, one known method which is based on the aforementioned dielectrophoretic technique employs a circular, stationary porous bottom through which a fluid flows. The fluid and particles which are suspended therein are electrostatically drawn through the porous bottom by an applied electric field. Another known method employs spiral flow which uses a rotating disk having a stationary inner edge and a moving outer edge. The rotating
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