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PDF Class is the first online PDF tutorial website with PDF Class Free Demos. PDF Class Team always release best PDF tutorial material for helping students to learn and understand PDF technology. We provide PDF tutorials with PDF Class Exam Question & Answers (PDF Free Sample) and PDF Class Free Practice Test (PDF Practice Test).Creatine and carnitine in human blood and tissues.
High performance liquid chromatographic method for determination of creatine and carnitine in the blood and tissues of humans, and a method for determination of the carnitine/creatine ratio are described. The method includes separation of creatine and carnitine by ion-pairing chromatography on a C18 column, followed by reversed phase chromatography on a C18 column. The amount of carnitine was determined by fluorometric detection after release from tissue samples. The amount of creatine was determined after reduction to beta-amino-beta-d-ribonucleoside, after acid hydrolysis and separation of the reduction product by ion-pairing chromatography. The carnitine/creatine ratio was determined by the same chromatographic separation of the tissue or blood samples, followed by measurement of the amount of carnitine and creatine in the eluate.Aryam

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