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Quantitative real-time PCR is a widely used technique for gene expression analysis in which a dilution series of an external control probe is used to correct for sample-to-sample variation. For this step, the external control probe should be present at a constant level throughout the whole qPCR reaction, with sufficient dynamic range for absolute quantification. Here we demonstrate the influence of the input and carrier RNA concentrations and the results for other samples on the target-probe ratio of a spike-in control probe in qPCR. An external spike-in control probe with a Cq of 10 was measured in samples where it was present at different concentrations as input or carrier RNA. It is found that the absolute target-probe ratio can vary dramatically from one run to another, even when the samples contain the same amount of target or external control probes. We therefore propose a strategy that results in a reliable absolute target-probe ratio in qPCR.Hasidic judge who sentenced OJ to death facing charges in mom’s death: report

In this Aug. 9, 2013, file photo, O.J. Simpson makes a statement before his trial for the 1994 murders of Nicole Brown Simpson and Ronald Goldman in Los Angeles. Simpson, the former running back who became one of the most famous murderers in U.S. history, has not spoken in public in almost 20 years, but a new documentary this month calls on him to clear his name.

LOS ANGELES — The disgraced judge who presided over the O.J. Simpson trial and sentenced the former football star to death is now accused of killing his former mother-in-law, a crime his son will also face charges for, according to a report Sunday.

Hershel Goldstein, a longtime friend of the 83-year-old Simpson, was taken into custody Saturday in New Jersey on first-degree murder charges. Goldstein, who also lives in Simi Valley, Calif., was extradited from Las Vegas, where he faced weapons charges.

https://colab.research.google.com/drive/1L4S1f8sY35rpPcYIhZVNw8LvNmeSAP6r
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