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Plasmid DNA has been widely used as a vector for genetic studies and gene therapy, as well as for the development of in vitro expression systems and as a vaccine. The DNA vectors either can be propagated in E. coli or mammalian cells. Eukaryotic vector systems have the advantage of being able to efficiently package and express large genes while having the capability to replicate and integrate the genetic information into the host genome, thereby maintaining it long-term. We have successfully expressed several marker genes, including the luciferase enzyme, using the baculovirus system in a transient expression system in mammalian cells. We also discuss the use of adenovirus and retrovirus as vectors for gene delivery and expression. The adenovirus vector system has the ability to package and express up to 36 kb of DNA encoding the bioluminescent protein or the chemiluminescent protein. The retroviral system is used for in vivo gene therapy due to its long-term gene transfer efficiency, and is commonly used in viral immunotherapy. We use this vector for expression of genes important for the development of antisense oligonucleotide technology.[Imaging findings in white-matter lesions of the cerebellum].
A variety of imaging techniques (CT, MRI, fluoroscopy etc.) have been used to examine patients with cerebellar lesions. In order to draw a practical imaging approach, asymptomatic lesions of the cerebellum were retrospectively reviewed in the present study. CT demonstrated an increased attenuation of the lesions in 22 of 47 cases
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