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Wnt/beta-catenin pathway is critical in embryogenesis, in the development of a variety of diseases and in the maintenance of stem cells. The pathway is deregulated in various cancers and affects gene transcription via modulation of a cascade of nuclear events including down-regulation of the expression of TCF/beta-catenin transcriptional repressors, up-regulation of target genes such as c-myc and survivin, and phosphorylation of beta-catenin. Conversely, in the absence of Wnt ligands, unphosphorylated beta-catenin is degraded via the ubiquitin-proteasome degradation system. Many studies have demonstrated that the activation of the Wnt/beta-catenin pathway and the accompanying up-regulation of target genes are important for the cell growth and angiogenesis in solid tumors. Using transgenic mice expressing Tcf4, beta-catenin and TCF-1, we analyzed the biological effects of the Wnt/beta-catenin pathway on angiogenesis and found that beta-catenin and TCF-1 promote endothelial tube formation, whereas their absence inhibits angiogenesis. These transgenic mice will provide useful tools to analyze the role of Wnt/beta-catenin pathway in angiogenesis.Make well-rounded resume
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[Does the relative position of the operated and the non-operated hindlimb influence the adductor longus muscle strength in the rat?].
Functional results of the Boerema procedure for treatment of drop foot in patients with polio sequelae are still disappointing. The problem of the adductor longus muscle weakness has been attributed to some specific position of the paralyzed leg during walking, and of the normal leg during the postoperative period. This paper therefore examines the role of the adductor longus muscle in hindlimb movements and its function in rats. Two supracondylar amputations were performed in each paw. The muscles from the amputated limbs were examined histologically and compared to the contralateral limb. Electromyography was performed to determine the pre- and postoperative status of the adductor longus muscle. We demonstrated large variations in the muscle force between the amputated and the non-amputated limb, but also a considerable degree of muscle contraction of the adductor longus muscle in the contralateral limb. There is no a relation between the degree of amputation of the limb and the muscular function. In view of these variations in the adductor longus muscle function, we conclude that a significant muscle function cannot be expected following a supracondylar amputation.